How to make primers for direct integration into markers of BY4741

Tutorial | I have been captivated by the idea of directly integrating a PCR product into the yeast genome. The integration usually is done at one of the auxotrophic marker loci in the lab strain. These regions contain the remains of the original gene or a crippled version of the gene. Since integration there requires homology, and the genes have been deleted using different strategies, I did set to make a set of primer fragments or overhangs that I could use to directly target to the deleted regions of, in my case, BY4741. You can find a notebook containing the annotated genomic sequences for a few markers, a Word file with the fragment themselves and the relevant literature here.